Rates of reactions of CDDP with MT obtained from HPLC-AAS data
The reactions at 37°C contained 33 μM CDDP and 74 to 83 μM MT (dialysates of Cd/Zn-thionein, Cd7-thionein, Zn7-thionein, and apo-thionein) in 4.62 mM NaCl and 10 mM Tris-NO3 (pH 7.4). The parameters in the linear fits of Figs. 4, 5a, and 6, A + Bt, are given; the rate constant k (M-1 s-1) is the slope B (μM-1 h-1).
MT | A | B | R2 | k |
|---|---|---|---|---|
| μM-1 | μM-1 h-1 | M-1 s-1 | ||
| Cd/Zn thioneina | 0.0062 | 0.00214 ± 0.00020 | 0.953 | 0.59 ± 0.06 |
| Cd/Zn thionein | -0.00279 | 0.00269 ± 0.00009 | 0.997 | 0.75 ± 0.03 |
| Cd7-thionein | 0.00158 | 0.00192 ± 0.00012 | 0.989 | 0.53 ± 0.03 |
| Zn7-thionein | -0.00128 | 0.00233 ± 0.00012 | 0.992 | 0.65 ± 0.03 |
|
Apo-thionein
|
-0.000021
|
0.00049 ± 0.00016
|
0.766
|
0.14 ± 0.04
|
↵ a Undialyzed Cd/Zn-thionein (from Sigma-Aldrich).