Carboxylic acids and 1-hydroxy-2-butanone detected in rat and mouse urine after treatment with 3-butene-1,2-diol a
Carboxylic acids and 1-hydroxy-2-butanone were extracted from urine, derivatized, and quantified as described under Material and Methods. Values shown represent the amounts of each compound prior to hydrolysis with β-glucuronidase/sulfatase. Total values after hydrolysis were not significantly different from values obtained prior to hydrolysis. Each value represents the mean ± S.D. of values obtained from at least three rats or mice.
Metabolite | Species | ||||||
|---|---|---|---|---|---|---|---|
|
SD Rats (μmol kg−1
BDDa)
|
B6C3F1 Mice (μmol kg−1
BDDa)
| ||||||
|
1420
|
2840
|
1420
|
2840
| ||||
| Propionic acid | 0.37 ± 0.75 | 4.85 ± 3.55 | 0.75 ± 0.66 | 0.82 ± 0.63 | |||
| 2-Ketobutyric acid | 0.14 ± 0.17 | 0.09 ± 0.06 | 0.63 ± 0.93 | 0.49 ± 0.90 | |||
| Crotonic acid | N.D. | 0.05 ± 0.05 | N.D. | 0.08 ± 0.16 | |||
| 1-Hydroxy-2-butanone | 1.09 ± 1.43 | 0.10 ± 0.05 | N.D. | 0.43 ± 0.48 | |||
|
Total amounts
|
1.60 ± 2.29
|
5.09 ± 3.55
|
1.38 ± 0.34
|
1.82 ± 0.88
| |||
N.D., not detected.
↵ a Doses of 1420 and 2840 μmol kg−1 BDD correspond to 125 and 250 mg kg−1, respectively.