Glucuronidation of anabolic androgenic steroids by recombinant human UGTs The formation of a given glucuronide was estimated as the ratio of peak areas of analyte and internal standard. A minus sign means that glucuronide formation, if to any extent, was below the detection limit, whereas each additional “+” indicates an approximate 10-fold increase in activity. Experimental conditions: 50 μM substrate, 5 mM UDPGA, 5 mM saccharic acid lactone, 0.5 mg/ml protein, and 2 h of incubation time
UGT | 5α-N | 5β-N | 5α-ME | MT | 5α-MT | 5β-MT | 5β-EPIM | TES | NAN | 5α-1-ME | 5α-A |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1A1 | + | ++ | — | — | — | — | — | ++ | ++ | — | +++ |
| 1A3 | ++ | ++ | +++ | — | ++ | ++ | + | ++ | ++ | + | +++ |
| 1A4 | +++ | +++ | ++++ | — | +++ | ++++ | — | +++ | +++ | ++++ | +++++ |
| 1A6 | — | — | — | — | — | — | — | — | — | — | — |
| 1A7 | — | — | — | — | — | — | — | — | — | — | — |
| 1A8 | — | — | — | — | — | — | — | ++ | +++ | ++ | +++ |
| 1A9 | — | — | — | — | — | — | — | + | ++ | ++ | +++ |
| 1A10 | — | ++ | — | — | — | — | ++ | +++ | ++++ | ++++ | ++++ |
| 2B4 | ++ | +++ | ++++ | — | ++ | ++ | ++++ | + | + | + | +++ |
| 2B7 | +++ | +++++ | ++++ | — | +++ | +++ | ++++ | + | ++ | +++ | ++++ |
|
2B15
|
—
|
—
|
—
|
—
|
—
|
—
|
—
|
+++
|
++
|
+++
|
+++++
|