Enzyme | Substrate | Product | Km a | n b | Vmax | CLintc |
---|---|---|---|---|---|---|
1A1 | 7-Ethoxyresorufin | Resorufin | 1.0 (0.4)d | 3.4 (0.3) | 3.4 | |
2B11 | DZ | NDZ | 5.4 (0.7) | 35.9 (1.7) | 6.6 | |
TMZ | OX | 39.6 (3.7) | 18.7 (0.8) | 0.5 | ||
Testosterone | 16α-OH-Testosterone | 4.6 (1.5) | 4.6 (0.4) | 1.0 | ||
Diclofenac | 4′-OH-Diclofenac | 5.3 (2.1) | 0.5 (0.02) | 0.01 | ||
2C21 | Testosterone | 16α-OH-Testosterone | 6.4 (1.2) | 17.3 (0.75) | 2.7 | |
(S)-Mephenytoin | 4′-OH-Mephenytoin | 131 (80) | 0.49 (0.14) | 0.004 | ||
Diclofenac | 4′-OH-Diclofenac | 93.9 (6.3) | 2.7 (0.08) | 0.03 | ||
2C41 | (S)-Mephenytoin | 4′-OH-Mephenytoin | 70 (16) | 0.14 (0.02) | 0.002 | |
Diclofenac | 4′-OH-Diclofenac | 155.3 (15) | 0.23 (0.01) | 0.001 | ||
2D15 | Bufuralol | 1′-OH-Bufuralol | 3.9 (0.4) | 6.7 (0.2) | 1.7 | |
Dextromethorphan | Dextrorphan | 0.72 (0.16) | 2.6 (0.1) | 3.6 | ||
3A12 | DZ | TMZ | 41.1 (2.1) | 1.6 | 12.2 (0.3) | 0.3 |
NDZ | OX | 115.4 (7.6) | 2.3 | 10.8 (0.5) | 0.1 | |
Testosterone | 6β-OH-Testosterone | 83 (12.4) | 21.5 (1.1) | 0.3 | ||
Testosterone | 16α-OH-Testosterone | 73.0 (27.0) | 2.2 (0.4) | 0.03 | ||
Testosterone | 16β-OH-Testosterone | 68.9 (4.4) | 2.3 (0.1) | 0.03 | ||
Testosterone | 2α/2β-OH-Testosterone | 154.0 (16.8) | 42.5 (2.8) | 0.3 | ||
Dextromethorphan | 3-Methoxymorphinan | N.D.e | N.D. | N.D. | ||
3A26 | Testosterone | 2α/2β-OH-Testosterone | 16.2 (2.4) | 2.3 | 1.4 (0.04) | 0.1 |
DLMs | 7-Ethoxyresorufin | Resorufin (low Km) | 0.8 (0.2) | 1.9 (0.3) | 2.4 | |
Resorufin (high Km) | 32.2 (12.4) | 6.6 (0.9) | 0.2 | |||
DZ | NDZ | 6.4 (0.3) | 6.3 (0.0) | 1.0 | ||
TMZ | 155.6 (14.2) | 1.5 | 6.5 (0.3) | 0.04 | ||
TMZ | OX | 75.8 (14.1) | 2.7 (0.3) | 0.04 | ||
NDZ | OX | 192.0 (35.6) | 1.5 | 1.7 (0.2) | 0.01 | |
Testosterone | 6β-OH-Testosterone | 73.6 (21.1) | 22.0 (2.3) | 0.3 | ||
Testosterone | 16α-OH-Testosterone | 13.8 (3.1) | 10.6 (0.5) | 0.8 | ||
Testosterone | 16β-OH-Testosterone | 87.2 (35.6) | 3.8 (0.6) | 0.04 | ||
Testosterone | 2α/2β-OH-Testosterone | 75.6 (15.8) | 41.8 (0.7) | 0.06 | ||
(S)-Mephenytoin | 4′-OH-Mephenytoin | 60 (15.6) | 2.3 (02) | 0.04 | ||
Diclofenac | 4′-OH-Diclofenac | 110 (12.5) | 0.41 (0.01) | 0.003 | ||
Bufuralol | 1′-OH-Bufuralol | 3.5 (0.3) | 3.5 (0.1) | 1.0 | ||
Dextromethorphan | Dextrorphan | 2.3 (0.6) | 4.3 (0.2) | 1.9 | ||
|
|
3-Methoxymorphinan
|
N.D.
|
|
N.D.
|
N.D.
|
↵ a Km (μM) and Vmax (nmol/min/nmol) were determined by either eq. 1 or eq. 2
↵ b n represents sigmoidicity of velocity curves from the Hill equation (eq. 2)
↵ c CLint = Vmax/Km
↵ d Values in parenthesis are the standard deviation and at least eight substrate concentrations around Km (duplicate in each) were utilized to determine the kinetic values
↵ e N.D., kinetic values not detectable because the velocity curves were not saturated with the concentrations of the substrate used