TABLE 1

HPLC-UV/ESP-MS-MS/MS analysis of trichostatin A and phase I metabolites

Peak
Retention Time
λ
M + H+
MS/MS
Proposed Structure
1 20.75 344 N.D.a N.D.
2 21.69 341 254 N.D. Not confirmed
3 22.56 341 289 289, 271, 259, 200, 164, 134 N-Demethyl trichostatin A
4 22.93 346 248 248, 230, 202, 174, 108 Not confirmed
5 23.90 340 273 273, 256, 255, 245, 228, 212, 166, 149, 134 N-Demethyl trichostatin A amide
6 23.97 344 250 250, 232, 108, MS3 of 232 → 204, 134 N-Demethyl dinor tetrahydro trichostatic acid
7 25.02 352 287 287, 270, 269, 177, 166, 149, 148, 122, 121 Trichostatin A amide
8 26.40 350 303 303, 285, 270, 178, 148, 122 Trichostatin A
9 27.02 357 262 244, 216, 188, 122; MS3 of 244 → 216, 188, 148 Not confirmed
10a 27.60 ~335-343 276 276, 258, 230, 151, 134, 108 N-Demethyl dihydro trichostatic acid
10b 27.68 287 287, 148 Trichostatin A amide isomer
10c 27.90 264 264, 246, 122, MS3 of 246 → 218, 148 Dinor tetrahydro trichostatic acid
11 28.32 341 274 134 N-Demethyl trichostatic acid
12 28.83 350 317 317, 299, 270, 196, 148, 122 Methyl trichostatin A
13 29.29 349 262 262, 244, 148, 122 Dinor dihydro trichostatic acid
14 30.54 350 234 N.D. Not confirmed
15 31.49 341 290 290, 272, 244, 187, 169, 141, 148, 122 Dihydro trichostatic acid
16 32.01 349 288 288, 270, 244, 242, 178, 167, 148, 122 Trichostatic acid
17
32.63
349
260
N.D.
Not confirmed

  • ↵ a N.D., mass spectrum not obtained.