TABLE 2

Apparent kinetic parameters of 5′ -DFCR formation from capecitabine in the purified enzyme, human liver cytosol, and microsomes

Capecitabine (0.05-3.0 mM) was incubated with the purified enzyme (4.4 μg/ml), cytosol (1.0 mg/ml), and microsomes (0.2 mg/ml) for 30 min. Kinetic parameters of cytosol and microsomes were obtained from our previous report (Tabata et al., 2004). Each data point represents the mean of duplicate determinations.