Apparent kinetic constants for 3-OH-BaP sulfotransferase and glucuronosyltransferase activity in human liver in the presence and absence of triclosan
Values are mean ± S.D. (n = 3).
Triclosan | Sulfonation | Triclosan | Glucuronidation | ||||
|---|---|---|---|---|---|---|---|
| Km | Vmax | Km | Vmax | ||||
| μM | μM | pmol·min−1·mg protein−1 | μM | μM | pmol·min−1·mg protein−1 | ||
| 0 | 0.057 ± 0.014 | 121 ± 36.7 | 0 | 15.1 ± 3.6 | 519 ± 137 | ||
| 0.5 | 0.054 ± 0.015 | 93.5 ± 39.9 | 5 | 24.1 ± 5.4 | 510 ± 171 | ||
| 1.0 | 0.060 ± 0.018 | 71.2 ± 29.2 | 10 | 31.3 ± 3.9 | 481 ± 149 | ||
| 2.0 | 0.074 ± 0.020 | 59.7 ± 22.6 | 20 | 27.9 ± 7.3 | 291 ± 138 | ||