CLint estimates determined from fresh and cryopreserved dog and human hepatocytes Results are expressed as the mean CLint ± standard deviation from five human and six dog donors. Results not expressing a S.D. are from two donors only. The CLint determinations were carried out as described under Materials and Methods. The CLint estimates reported in Table 1 represent a different donor pool from the one reported in this table, which can result in different mean values for the same substrate.
Compounds | CLint | ||||||
|---|---|---|---|---|---|---|---|
| Human hepatocytes | Dog hepatocytes | ||||||
| Fresh | Cryo | Fresh | Cryo | ||||
| μl/min/106 cells | μl/min/106 cells | ||||||
| Dextromethorphan | 16 ± 8 | 15 ± 6 | 64 ± 13 | 56 ± 29 | |||
| Diazepam | <1 | <1 | >150 | >150 | |||
| Diclofenac | 47 ± 22 | 44 ± 14 | 12 ± 5 | 11 ± 4 | |||
| Diltiazem | 13 ± 4 | 13 ± 3 | 29 ± 10 | 20 ± 9 | |||
| Ethinylestradiol | 7 ± 2 | 5 ± 3 | 69 | 83 | |||
| Gemfibrozil | 24 ± 5 | 20 ± 5 | 13 ± 1 | 11 ± 2 | |||
| Imipramine | 4 | 7 ± 3 | 32 ± 10 | 45 ± 26 | |||
| Ketoprofen | 4 ± 2 | 1 ± 1 | 4 ± 0.4 | 3 ± 2 | |||
| Metoprolol | 7 ± 6 | 10 ± 3 | 11 ± 4 | 13 ± 12 | |||
| Midazolam | 14 ± 8 | 11 ± 3 | 125 | 114 | |||
| Naproxen | 5 ± 2 | 3 ± 3 | 3 ± 1 | 3 ± 1 | |||
| Propofol | 107 ± 26 | 92 ± 36 | >150 | >150 | |||
| Propranolol | 15 ± 5 | 19 ± 3 | 38 ± 10 | 33 ± 16 | |||
| Verapamil | 42 ± 8 | 43 ± 17 | 38 ± 12 | 35 ± 17 | |||