Genotyping methods used for the CYP2B6 polymorphisms by SNaPshot or RFLP
Polymorphism | Forward Primer 5′-3′ | Reverse Primer 5′-3′ | Annealing Temperature | Product Size | SNaPshot (Reverse Primer 5′-3′) or RFLP (Restriction Enzyme) |
|---|---|---|---|---|---|
| °C | bp | ||||
| -8427T>C | AGTCCATGGTGGCATCTTTC | TTCCCCACCCTCTTCTATCC | 60 | 485 | SNaPshot (CCCCACATCCATGTGGATTAGACAC) |
| -8207G>C | AGTCCATGGTGGCATCTTTC | TTCCCCACCCTCTTCTATCC | 60 | 485 | SNaPshot (GGGTCTGTCTCCATCACTGCTGTGAGC) |
| -2320T>C | CCTCCCCTTTTGAAAATC | GCAACATGGATACAACTGG | 60 | 361 | SNaPshot (GCATTATCAGGGGAAATTCAGCCAG) |
| -1778A>G | GGACAATGTAGCCCCAACC | TTGCAAAACGTGGGTGTG | 61.7 | 465 | SNaPshot (TTCCTGAACCAAGGGCCTCGGTCTTGC) |
| -1456T>C | TTTTGCAAAGATGGACTGACC | TGTGTTGGTGGATCTCCTAGC | 65 | 481 | RFLP (AccI) |
| -1186C>G | TTTTGCAAAGATGGACTGACC | TGTGTTGGTGGATCTCCTAGC | 65 | 481 | RFLP (BseRI) |
| -750T>C | GCAGTAGGCCAAATTTGATTG | TTGTGTGTTGTGGAGGTGGT | 60 | 410 | SNaPshot (ATCTGTACTAAAAACACAAAAATTA) |
| 415A>G | TCCGGGGATATGGTGAGA | CCGTGCACCTGCATCTCT | 62.4 | 358 | SNaPshot (TGAATCCGCTCCTCCACACTCCGCT) |
| 15582C>T | TCAAATTGCATCTGCCTCAC | TCTGCGTTTCTTCCAAGGAT | 60 | 480 | SNaPshot (GAGGGCCCCTAGGAAGAAGCAGCAG) |