Effects of MgCl2 and substrates on N-phenylprotoporphyrin regioisomer formation from CYP3A4
CYP3A4 substrates were dissolved in methanol, and the final concentration of methanol was 1% in all conditions.
Percentage of the Total under Each Condition | Total (Percentage of Control) | |||||||
|---|---|---|---|---|---|---|---|---|
| NB | NA | NC | ND | |||||
| Control | 7 (10,4)a | 72 (68,76) | 5 (6,5) | 16 (17,15) | 100 (106,94) | |||
| + MgCl2 (10 mM) | 7 (5,8) | 72 (74,69) | 6 (8,5) | 15 (13,17) | 95 (92,97) | |||
| + Midazolam (25 μM) | 5 (5,6) | 74 (72,76) | 7 (8,7) | 13 (15,12) | 136 (128,143) | |||
| + Midazolam (250 μM) | 8 (6,11) | 72 (75,70) | 8 (8,7) | 11 (10,13) | 161 (152,169) | |||
| + 7-BFC (100 μM) | 5 (6,5) | 72 (67,76) | 9 (13,5) | 14 (14,14) | 103 (100,106) | |||
| + Mifepristone (100 μM) | 10 (9,11) | 66 (67,66) | 9 (10,8) | 15 (14,15) | 182 (190,173) | |||
| + Diazepam (250 μM) | 9 (12,7) | 63 (60,66) | 8 (7,8) | 20 (21,18) | 140 (144,136) | |||
↵ a All values are the mean of duplicate determinations, which are shown in parentheses.