TABLE 4

Expression levels of CYP3A4, CYP3A5, reductase, and b5 in selected human liver microsomes with equivalent CYP3A4 expression (group 1, predominantly CYP3A4; group 2, CYP3A4 + CYP3A5)

Two-tailed unpaired t test used to compare groups 1 and 2.

Liver
Age
Gender
CYP3A4
CYP3A5
Total 3A
Reductase Activity
Reductase
b5
pmol/mg nmol/min/mg pmol/mg nmol/mg
Group 1
HL-102 21 M 85 3.9 88.9 124 41 0.41
HL-103 15 F 79 4.0 83 127 42 0.52
HL-109 46 M 102 2.1 104 127 42 0.31
HL-111 28 M 95 4.7 99.7 119 40 0.47
HL-115 52 F 52 2.5 54.5 127 42 0.38
HL-124 51 F 171 5.0 176 143 48 0.34
HL-129 36 F 133 3.9 137 168 56 0.41
HL-132 50 F 59 2.5 61.5 107 36 0.38
HL-168 43 M 72 4.0 76 159 53 0.41
HL-171 47 F 80 3.4 83.4 135 45 0.33
Mean ± S.D. 39 ± 13 93 ± 36 3.6 ± 1.0 96.4 ± 36 134 ± 18 44.5 ± 6.2 0.40 ± 0.06
Group 2
HL-141 59 M 90 291 381 164 55 0.45
HL-121 59 F 75 64 138 128 43 0.31
HL-119 24 M 106 101 207 144 48 0.37
HL-158 59 M 102 103 205 155 52 0.32
HL-162 56 F 48 60 108 137 46 0.26
HL-144 68 F 187 164 351 169 56 0.45
HL-163 55 M 106 125 231 147 49 0.35
HL-108 42 F 57 89 146 130 43 0.36
HL-125 32 M 72 71 143 125 42 0.37
HL-147 70 F 86 101 187 125 42 0.34
Mean ± S.D.
52 ± 15*
93 ± 39
117 ± 68***
210 ± 91**
142 ± 16
47.6 ± 5.3
0.36 ± 0.06

  • *p < 0.05; ** p < 0.01; *** p < 0.001.