Sulfation of α-OHTAM stereoisomers catalyzed by rat STa
Calculation of kcat values was based on 33,120 as the subunit molecular mass of STa.
Stereoisomer | Km | Vmax | kcat/Km |
|---|---|---|---|
| μM | nmol/min/mg | min-1mM-1 | |
| E-(±)-α-OHTAM | 48.2 ± 7.9 | 24.2 ± 1.1 | 16.6 ± 2.8 |
| E-(+)-α-OHTAM | 36.0 ± 2.5 | 34.6 ± 0.6 | 31.8 ± 2.3 |
| E-(-)-α-OHTAM | 0a | ||
| Z-(±)-α-OHTAM | 0b | ||
| Z-(+)-α-OHTAM | 0b | ||
| Z-(-)-α-OHTAM |
| 0b |
|
a,b No detectable rate of reaction at substrate concentrations of 80, 200, 400a, and 200 μMb.