TABLE 4

Rates of DHEA metabolites formed from baculovirus-expressed P450 Metabolic assays were performed in triplicate in 2-ml reaction mixtures containing P450 baculovirus (∼0.4 nmol/ml), NADPH-regenerating system, and 50 μM DHEA and incubated at 37°C for 10 min in a shaking water bath. Reactions were terminated at 5 min and 10 min. Ethyl acetate extracts were examined by GC/MS. The results are expressed as the average of triplicate experiments of at least two reactions in which the S.D. varied by ≤ 5%. The rates of metabolism during the linear portion of the reaction are statistically different from a reaction in the absence of baculovirus preparation (^*p < 0.05 or ^**p < 0.01).