Kinetic parameters for the formation of glucuronides from 14C-ezetimibe by human UGT Supersomes
Kinetic parameters were determined by GraFit 4.04 program (Erithacus Software).
Kinetic Parameter | UGT1A1 | UGT1A3 | UGT2B7 |
|---|---|---|---|
| Phenolic/benzylic glucuronide | Phenolic | Phenolic | Benzylic |
| Km (μM) | 64.3 ± 17.2 | 41.7 ± 15.4 | 20.9 ± 4.05 |
| Vmax (nmol/mg protein/min) | 0.71 ± 0.09 | 0.48 ± 0.07 | 0.05 ± 0.004 |
| Vmax/Km (ml/mg protein/min)a | 0.011 | 0.011 | 0.002 |
↵ a Intrinsic clearance