TABLE 1

The parameters GI₅₀, TGI, and LC₅₀ obtained for cis-AVTP in the NCI anticancer screen Parameters below or above the highest or lowest drug concentration used are listed as <0.01 or >100, respectively. All values are given in micromolar concentration (μM).

Cell Line	Tissue Type	GI₅₀	TGI	LC₅₀	Cell Line	Tissue Type	GI₅₀	TGI	LC₅₀
CCRF-CEM	Leukemia	0.21	2.17	>100	LOX IMVI	Melanoma	0.03	2.23	>100
HL-60(TB)	Leukemia	0.49	4.95	>100	MALME-3M	Melanoma	N.D.	N.D.	N.D.
K-562	Leukemia	0.09	>100	>100	M14	Melanoma	0.07	1.89	7.75
MOLT-4	Leukemia	0.36	>100	>100	SK-MEL-2	Melanoma	0.48	8.22	40.7
RPMI-8226	Leukemia	0.12	1.67	>100	SK-MEL-28	Melanoma	1.84	4.19	9.58
SR	Leukemia	0.17	>100	>100	SK-MEL-5	Melanoma	1.30	3.24	8.07
					UACC-257	Melanoma	2.40	27.4	>100
A549/ATCC	NSCL	6.94	24.3	69.5	UACC-62	Melanoma	0.23	1.10	5.88
HOP-62	NSCL	0.40	21.2	88.9
HOP-92	NSCL	1.69	5.45	32.9	IGR-OV1	Ovarian	2.00	7.72	29.8
NCI-H23	NSCL	1.48	4.58	27.8	OVCAR-3	Ovarian	0.03	0.47	8.67
NCI-H322M	NSCL	2.49	15.6	68.8	OVCAR-4	Ovarian	0.51	4.06	25.6
NCI-H460	NSCL	0.68	8.71	38.6	OVCAR-5	Ovarian	1.31	2.94	6.57
NCI-H522	NSCL	N.D.	N.D.	N.D.	OVCAR-8	Ovarian	1.53	5.93	>100
					SK-OV-3	Ovarian	0.11	5.04	33.3
COLO 205	Colon	0.46	1.70	4.74
HCT-116	Colon	0.51	1.89	4.73	786-0	Renal	0.37	3.48	24.9
HCT-15	Colon	1.88	5.98	41.3	ACHN	Renal	1.99	3.89	7.59
HT29	Colon	0.73	2.85	9.23	CAKI-1	Renal	0.11	0.55	2.85
KM12	Colon	3.35	27.2	>100	SN12C	Renal	2.03	5.75	28.1
SW-620	Colon	1.52	>100	>100	TK-10	Renal	0.03	0.64	13.9
					UO-31	Renal	0.60	1.85	4.48
SF-268	CNS	<0.01	0.24	27.7
SF-295	CNS	0.56	11.2	57.8	PC-3	Prostate	2.05	10.2	85.1
SF-539	CNS	0.65	2.86	11.8	DU-145	Prostate	1.32	3.97	24.4
SNB-19	CNS	3.66	18.2	76.3
SNB-75	CNS	4.19	18.6	49.1	NCI/ADR-RES	Breast	0.51	2.79	>100
U251	CNS	2.74	10.2	56.3	MDA-MB-231/ATCC	Breast	1.27	4.56	>100
					HS 578T	Breast	0.46	8.79	>100
					MDA-MB-435	Breast	0.56	2.75	9.80
					MDA-N	Breast	0.55	4.48	>100
					BT-549	Breast	1.24	3.48	9.78

N.D., cis-AVTP cytotoxicity was not determined in this cell line.