TABLE 2

Primers used for amplification of the nine exons of the CYP2C9 gene (PCR I) and for sequencing The location of each primer is given in accordance with LokusLink Version NT_030059.8 GI:27498938.


Primer Name

Primer Position

Primer Sequence (5′ to 3′)
E1F 37,832-37,853 CCAACCAAGTACAGTGAAACTG
E1Ra 38,491-38,509 TGAAGAAGGAACACAGCT
E2 + 3F 40,997-41,021 CCTGCTGAATATGTTGATGTGAGTA
E2 + 3Ra 41,830-41,849 AGCTAACAACCAGGACTCAT
E4Fb 46,861-46,879 TGTGATTCCCTCTGAAACA
E5F 48,259-48,280 GGAGGTCTGAGTCTAGGAAATG
E5Ra 48,771-48,790 TGCTTCTCAAGCATTACTGA
E6Fb 71,243-71,262 GCTGTGTGACTCAGCTAGCT
E6Ra,b 71,955-71,974 AGTAGCTGGGATTACAGCCA
E7F 80,404-80,424 CATTTGTGCATCTGTAACCA
E7Ra 80,931-80,950 ACAGGTCCTAGTGTCTGGCA
E8F 85,217-85,236 TTGCAGGGCACTTTAGCAAG
E8Ra 85,747-85,768 TGGATTAACTCCCCAAAGTCCA
E9F 88,056-88,074 ATCCATCCACTCATCCATC
E9Ra
88,576-88,594
GAAACATAGGAAACTCTCCG
  • a Reverse primers

  • b Elongase Enzyme Mix (Invitrogen) was used