TABLE 1

Protein amount, incubation time, and substrate concentration ranges used for the measurement of 4MU and 1NP glucuronidation

Isoform
4MU
1NP
Protein Amount
Incubation Time
Concentration Range
Protein Amount
Incubation Time
Concentration Range
μg/incubation min μM μg/incubation min μM
1A1a 200 120 20-750 200 120 50-1000
1A3 100 75 20-2000 300 120 100-3000
1A4 300 120 10-1000 300 120 10-1000
1A6 1.5 30 10-600 0.5 60 0.5-20
1A7 5 10 1-100 100 30 0.5-20
1A8 30 30 20-2000 30 30 25-600
1A9 15 15 1-60 200 60 0.5-15
1A10 12.5 30 5-140 12.5 30 1-50
2B7 150 120 50-1500 300 120 25-800
2B15 500 120 50-1500 500 120 10-1000
2B17
300
120
200-2500
100
120
10-1000

  • ↵ a Conditions for UGT1A1 from Sorich et al. (2002).