TABLE 4

In vitro clearance for 4MU and 1NP glucuronidation by human UGT isoforms

Isoform
CLinta or CLmaxb
4MU
1NP
μl/min · mg protein
1A1 2.72a 0.77b
1A3 1.1a 0.06a
1A4 N.D. N.D.
1A6 1320a 3602b
1A7 2553a 27b
1A8 12.6a 6.1b
1A9 1128a 13.1b
1A10 438a 673a
2B7 0.52b 0.19b
2B15 0.10a N.D.
2B17
0.01a
N.D.

  • N.D., not determined.

  • ↵ a CLint calculated as Vmax/Km for both Michaelis-Menten and substrate inhibition kinetics.

  • ↵ b CLmax calculated using eq. 4 (Materials and Methods, Data Analysis).