Volume | α-NA Activitya | Total Activity | Protein | Total Protein | Specific Activity | Fold Purification | |
---|---|---|---|---|---|---|---|
ml | U/ml | U | mg/ml | mg | U/mg | ||
Cell Lysate 100 | 38 | 1.26 | 48 | 9.4 | 356 | 0.13 | 1 |
Concanavalin A concentrate | 0.7 | 5.6 | 3.9 | 4.0 | 2.8 | 1.4 | 10 |
Prep Cell concentrate | 0.4 | 9.3 | 3.9 | 1.6 | 0.67 | 5.8 | 44 |
↵ a The activity was monitored by α-napthylacetate (α-NA) hydrolase with Fast Violet Blue. One unit is defined as 1 μmol of product formed per minute