Volume | 4-MUAa Activity | Total Activity | Protein | Total Protein | Specific Activity | |
---|---|---|---|---|---|---|
ml | U/ml | U | mg/ml | mg | U/mg | |
Liver homogenate | 220 | 5.29 | 1164 | 18.8 | 4136 | 0.28 |
Concentrate after DE52 | 123 | 8.6 | 1057 | 16.7 | 2054 | 0.51 |
Concanavalin A concentrate | 4 | 186 | 742 | 8.8 | 35 | 21.2 |
Prep Cell concentrate CES1A1 | 2 | 77 | 154 | 11.6 | 23.2 | 6.6 |
Prep Cell concentrate CES2 | 0.5 | 814 | 407 | 6.8 | 3.4 | 120 |
↵ a The 4-methylumbelliferyl acetate (MUA) activity was measured at 37°C in 90 mM KH2PO4, 40 mM KCl, pH 7.4 buffer with 0.5 mM 4-methylumbelliferyl acetate. The formation of product, 4-methylumbelliferone, was monitored spectrophotometrically at 350 nm