TABLE 2

Effect of hypericin (Hpc) and hyperforin (Hpf) on CYP2C9 and CYP3A4 activity

Culture ID	CYP3A4 - Testosterone					CYP2C9 - Flurbiprofen
Culture ID		HH906	HH913	HH921	HH913	HH919	HH926
		250 μM			100 μM
DMSO	0.10 ± 0.01	0.26 ± 0.01	0.19 ± 0.01	0.12 ± 0.03	0.16 ± 0.03	0.07 ± 0.01
Rif 10 μM	0.96 ± 0.09^*	1.67 ± 0.01^*	1.43 ± 0.23^*	0.27 ± 0.04^*	0.35 ± 0.03^*	0.20 ± 0.03^*
Hpc 1.0 μM	0.16 ± 0.02	0.24 ± 0.03	0.25 ± 0.06	0.13 ± 0.03	0.17 ± 0.01	0.08 ± 0.00
Hpc 2.5 μM	0.16 ± 0.02	0.33 ± 0.05	0.16 ± 0.04	0.13 ± 0.02	0.18 ± 0.02	0.10 ± 0.02
Hpf 0.2 μM	0.33 ± 0.03^*	0.52 ± 0.11^*	0.18 ± 0.05	0.18 ± 0.01^*	0.30 ± 0.08^*	0.14 ± 0.02^*
Hpf 1.0 μM	0.79 ± 0.07^*	0.84 ± 0.06^*	0.92 ± 0.15^*	0.19 ± 0.01^*	0.24 ± 0.03^*	0.12 ± 0.01^*

Rif, rifampicin. ^*. significantly different from DMSO-treated cells, p < 0.05.