TABLE 1

Probe used in electromobility shift assay experiments Double-stranded probes were generated for EMSA experiments corresponding to the consensus-binding region for each factor, and the mutated sequence were generated via SDM. Sense strand only is shown for clarity with the putative binding sites underlined, and mutated bases are indicated in bold.


Factor

Wild-Type Element Probe

Mutated Element Probe
PXR TATGAACTCAAAGGAGGTCAGT TATGTTCTCAAAGGAGAACAGT
Sp1 GAAGCCTCTTCCAACTGC GAAGCCTCTTTTAACTGC
HNF3 GATTGAGTTGTTTATGATAC GATTGAGTTGTGGATGATAC
C/EBPα
GTGATTCTTTGCCAACTTCC
GTGATTCGAGGCCAACTTCC