Effect of ETM and KTZ on the sulfoxidation of ABZ by liver microsomes from cattle and sheep Data are the mean (±S.D.) of 12 determinations.
Incubated Substrates | (-) ABZSO | (+) ABZSO | Ratio (±)a | % Inhibition of (-) ABZSO Production | |
|---|---|---|---|---|---|
| nmol · min-1 · mg-1 | |||||
| Cattle Liver Microsomes | |||||
| ABZ (control) | 0.19 ± 0.03 | 0.49 ± 0.12 | 2.63 ± 0.62 | N.A. | |
| ABZ + ETM (40 μM) | 0.17 ± 0.03 | 0.53 ± 0.09 | 3.10 ± 0.13 | N.A. | |
| ABZ + ETM (250 μM) | 0.16 ± 0.03* | 0.52 ± 0.08 | 3.33 ± 0.18** | 16 | |
| ABZ + ETM (500 μM) | 0.15 ± 0.03* | 0.55 ± 0.07 | 3.60 ± 0.35** | 21 | |
| Sheep Liver Microsomes | |||||
| ABZ (control) | 0.11 ± 0.03 | 0.42 ± 0.11 | 4.11 ± 1.43 | N.A. | |
| ABZ + KTZ (40 μM) | 0.08 ± 0.01* | 0.42 ± 0.06 | 5.31 ± 0.55* | 27 | |
| ABZ + KTZ (100 μM) | 0.07 ± 0.01** | 0.40 ± 0.07 | 5.60 ± 0.67** | 36 | |
| ABZ + KTZ (200 μM) | 0.07 ± 0.01** | 0.39 ± 0.08 | 5.29 ± 0.95* | 36 | |
N.A., not applicable.
Values are significantly different from those observed in ABZ control incubations at *p < 0.05, **p < 0.01, and ***p < 0.001.
↵ a The mean (±S.D.) ratio between the rate of (+) ABZSO and (-) ABZSO production found in each individual assay