Characterization of the site-directed mutants of AST IV by MALDI-TOF mass spectrometry
Enzyme | Tryptic Fragment | Calculated Mass | Observed Mass |
|---|---|---|---|
| F77A | VPALEFK | 803.0 | 803.2 |
| (Wild-type) | (VPFLEFK) | (879.1) | (879.4) |
| F138A | DVVVSYYNAYNMAK | 1636.9 | 1636.7 |
| (Wild-type) | (DVVVSYYNFYNMAK) | (1713.0) | (1712.6) |
| Y236A | ENCMTNATTIPTEIMDHNVSPFMI | 2756.2 | 2753.2 |
| (Wild-type) | (ENCMTNYTTIPTEIMDHNVSPFMI) | (2845.3) | (2845.7) |