TABLE 1

Oligonucleotide primer used in the gene expression study PCR conditions: predenaturation 95°C, 120 s; denaturation 94°C, 60 s; annealing (see table) for 60 s; and extension 72°C for 60 s.

Accession Number	Gene	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Product Length	PCR Cycles	Annealing Temperature
				base pairs		°C
	Cytochrome P450 genes
X00469	CYP1A1	CTGGTTCTGGATACCCAGCTG	CCTAGGGTTGGTTACCAGG	331	35	57
X01031	CYP1A2	GTCACCTCAGGGAATGCTGTG	GTTGACAATCTTCTCCTGAGG	236	35	57
NM_012692	CYP2A1/2	CACAGGGCAGCTCTATGACA	CAGACCCAGCAAAGAAGAGG	275	31	57
L00316	CYP2B1/2	GAGTTCTTCTCTGGGTTCCTG	ACTGTGGGTCATGGAGAGCTG	549	29	57
U33173	CYP2C11	CTGCTGCTGCTGAAACACGTG	GGATGACAGCGATACTATCAC	248	35	57
M20131	CYP2E1	CTCCTCGTCATATCCATCTG	GCAGCCAATCAGAAATGTGG	473	30	57
U39943	CYP2J3	GGCATGCCCTTAATCAAAGA	AGCCTCAGCATCTCCTGAAA	364	30	57
X64401	CYP3A1	ATCCGATATGGAGATCAC	GAAGAAGTCCTTGCTTGC	579	35	57
X79320	CYP3A2	CGACTTGGAACCCATAGAC	GGCTTAGGGAGATTTGACATG	116	35	57
M57718	CYP4A1	GGTGACAAAGAACTACAGC	AGAGGAGTCTTGACCTGCCAG	344	35	57
	17β-Hydroxysteroid dehydrogenase genes
NM_012851	17bHSD1	CCTCTTTGCCCACTATCAGC	GGAGACAAATGAGGGCTCTG	299	31	57
NM_024391	17bHSD2	TGTGCTGGTGACAGGTG	ACGGTCATGGGAATGAGTTC	295	33	55
NM_054007	17bHSD3	CCTCCGTAGTCAAGATGACA	CAAGGCAGCCACAGGTTTCA	333	33	55
NM_024392	17bHSD4	TATGCCCTGGCTTTTGCAGA	CAGTCTTCATCACTTATCCT	275	30	57
NM_012584	3β-Hydroxysteroid dehydrogenase 1	ATTCCAGTTGGAGCCTTCCT	CCCTCTTCCCATCATTGAGA	292	33	55
J05035	5α-Reductase	CCTTCCTGTTCTGCACCTTC	TGCAAACACTACACCCTGGA	308	33	55
M23264	Androgen receptor	GGTAATATCCGAAGGCAGCA	CAGCCCAGACTCTCACCTTC	381	29	57
NM_012689	Estrogen receptor α	GCACAAGCGTCAGAGAGATG	ATCTCCAACCAGGCACACTC	360	29	57
M19533	Cyclophilin A	CTTGCCATTCCTGGACCCAA	TTTCGTGCTCTGAGCACTGG	347	26	57