TABLE 4

Stabilities of stock solutions of the 12 analytes used in these assays

Assay
Analyte
Storage Solvent (CH3CN/H2O)
Storage Temperature
Established Stabilityb
°C days
CYP1A2 Acetaminophen 95/5 -10 109
CYP2A6 7-Hydroxycoumarin 50/50 4 39
CYP2B6 Hydroxybupropion 95/5 -10 43
CYP2C8 Desethylamodiaquine 50/50 4 64
CYP2C9 4′-Hydroxydiclofenac 95/5 -10 42
CYP2C9 Hydoxymethyltolbutamide 50/50 4 297
CYP2C19 4′-Hydroxymephenytoin 95/5 -10 41
CYP2D6 Dextrorphan 95/5 -10 69
CYP2E1 6-Hydroxychlorzoxazone 95/5 4 146
CYP3A Dehydrofelodipine 90/5/5a -10 43
CYP3A 1′-Hydroxymidazolam 75/5/20a -10 50
CYP3A
6β-Hydroxytestosterone
95/5
-10
59

  • ↵ a (CH3CN/CH3OH/H2O)

  • ↵ b Stability is defined as the period of time at which it has been demonstrated that the concentration of the stock solution has not deviated more than 10% of the value measured on the day when the stock was prepared