TABLE 5

Summary of analytical method performance characteristics for 12 human cytochrome P450 assays


Analyte

Enzyme

QC Concentrations

Mean Accuracy

Mean Precision
Low QC
Mid QC
High QC
Low QC
Mid QC
High QC
Low QC
Mid QC
High QC
nM % %
Acetaminophen CYP1A2 50.0 2000 8000 108 105 103 8.17 5.06 5.47
7-Hydroxycoumarin CYP2A6 30.0 400 1600 103 101 98.1 4.24 3.14 2.56
Hydroxybupropion CYP2B6 15.0 200 1600 104 107 105 7.57 3.67 6.85
Desethylamodiaquine CYP2C8 30.0 200 1200 101 101 97.1 3.76 2.21 3.70
4′-Hydroxydiclofenac CYP2C9 30.0 800 1600 105 105 106 10.2 7.84 9.23
Hydroxytolbutamide CYP2C9 30.0 400 4000 103 98.9 100 8.05 7.29 2.31
4′-Hydroxymephenytoin CYP2C19 150 200 8000 100 98.1 97.8 5.57 4.37 4.04
Dextrorphan CYP2D6 3.00 200 800 104 101 97.2 5.68 4.47 4.10
6-Hydroxychlorzoxazone CYP2E1 200 800 1600 99.5 97.7 98.9 5.85 4.27 5.04
Dehydrofelodipine CYP3A 15.0 200 1600 103 102 102 3.49 3.60 2.79
1′-Hydroxymidazolam CYP3A 3.00 200 800 104 96.6 97.2 7.03 4.74 4.46
6β-Hydroxytestosterone
CYP3A
90.0
2000
8000
99.4
97.9
98.1
6.00
5.19
8.53
  • QC. quality control.

    %Accuracy = 100 · [Analyte]measured/[Analyte]nominal.

    %Precision = 100 · S.D./[Analyte]measured, average.