TABLE 6

Interday precision for activity for assays in pooled human liver microsomes


Enzyme

Activity

Substrate Concentration

N

Interassay Precision of Reaction Velocity
μM %
CYP1A2 Phenacetin O-deethylase 50 47 4.7
CYP2A6 Coumarin 7-hydroxylase 1.0 28 9.8
CYP2B6 Bupropion hydroxylase 80 64 5.5
CYP2C8 Amodiaquine N-deethylase 1.9 97 5.3
CYP2C9 Diclofenac 4′-hydroxylase 4.0 44 5.6
CYP2C9 Tolbutamide hydroxylase 150 34 6.2
CYP2C19 (S)-Mephenytoin 4′-hydroxylase 60 63 8.4
CYP2D6 Dextromethorphan O-demethylase 5.0 60 5.6
CYP2E1 Chlorzoxazone 6-hydroxylase 75 38 7.6
CYP3A Felodipine dehydrogenase 3.0 87 7.3
CYP3A Midazolam 1′-hydroxylase 2.5 66 8.1
CYP3A
Testosterone 6β-hydroxylase
50
40
6.9