Kinetic parameters for MPA glucuronidation by UGTs
UGT | Concentration | Ether | Acyl | Km | Vmax | Km/Vmax |
|---|---|---|---|---|---|---|
| μM | % | % | μM | pmol/mg protein/h | h/mg protein | |
| UGT1A7 | 400 | 85 ± 1.6 | 15 ± 0.29 | |||
| 800 | 83 ± 1.2 | 17 ± 0.13 | 375 | 18.23 × 103 | 20.57 × 103 | |
| 1600 | 82 ± 1.3 | 18 ± 0.30 | ||||
| UGT1A8 | 400 | 85 ± 1.1 | 15 ± 0.32 | |||
| 800 | 84 ± 1.1 | 16 ± 0.38 | 500 | 4.48 × 103 | 111 × 103 | |
| 1600 | 80 ± 1.5 | 20 ± 0.36 | ||||
| UGT1A9 | 400 | 82 ± 1.0 | 18 ± 0.15 | |||
| 800 | 83 ± 1.0 | 17 ± 0.17 | 250 | 9.18 × 103 | 27 × 103 | |
| 1600 | 81 ± 1.0 | 19 ± 0.26 | ||||
| UGT1A10 | 400 | 66 ± 1.7 | 34 ± 0.7 | |||
| 800 | 62 ± 1.5 | 38 ± 0.6 | 550 | 4.00 × 103 | 137 × 103 | |
|
| 1600 | 58 ± 1.6 | 42 ± 0.8 |
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