CYP1A1a | CYP1A2 | CYP1B1 | Cyp1b1 | |
---|---|---|---|---|
Km(app) (μM) | 34.1 | 95.2 | 29.8 | 500.0 |
Vmax (nmol min-1) | 0.23 | 1.11 | 0.07 | 0.02 |
kcat = Vmax/Et (min-1) | 11.60 | 7.40 | 0.48 | 0.13 |
Catalytic efficiency [kcat/Km(app)] (mM-1 min-1) | 340.0 | 77.7 | 16.5 | 0.3 |
Terminal HETEs (% of total) | 75.40 ± 2.23 | 24.12 ± 0.45 | 21.63 ± 2.71 | 10.94 ± 4.45 |
20- and 19-HETE | 39.12 ± 2.82 | 10.22 ± 0.29 | 5.04 ± 1.06 | N.D. |
16,17,18-HETE | 36.28 ± 0.58 | 13.90 ± 0.74 | 16.59 ± 1.73 | 10.94 ± 4.45 |
Midchain HETEs (% of total) | 18.33 ± 0.92 | 22.52 ± 0.38 | 54.46 ± 2.50 | 37.16 ± 6.15 |
15-HETE | 9.89 ± 0.14 | 5.28 ± 0.35 | 12.42 ± 1.42 | 5.05 ± 1.58 |
11-HETE | 2.73 ± 0.47 | 3.31 ± 0.31 | 7.16 ± 0.74 | 3.37 ± 1.62 |
12- and 8-HETE | 3.29 ± 0.15 | 3.78 ± 0.16 | 9.77 ± 1.12 | 12.67 ± 1.86 |
5-HETE | 2.41 ± 0.15 | 10.16 ± 0.25 | 25.10 ± 0.37 | 16.13 ± 4.54 |
Epoxides (EETs) (% of total) | 5.23 ± 2.31 | 52.46 ± 0.98 | 23.57 ± 0.72 | 50.34 ± 5.04 |
14,15-EET | 3.35 ± 1.47 | 12.18 ± 1.04 | 6.87 ± 0.72 | 26.7 ± 1.81 |
11,12-EET | 1.12 ± 0.62 | 12.43 ± 1.87 | 5.20 ± 1.11 | N.D. |
8,9-EET | 0.23 ± 0.16 | 11.99 ± 1.51 | 4.91 ± 0.35 | 8.63 ± 2.13 |
5,6-EET | 0.72 ± 0.20 | 15.88 ± 1.35 | 6.78 ± 1.25 | 15.1 ± 2.77 |
N.D., none detected; Et, total amount of enzyme in the assay.
↵ a Supersomes