TABLE 3

Influence of CO in the atmosphere on the quantity and enantiomer composition of NNAL obtained on microsomal NNK reduction

NNK (250 μM) was incubated with a NADPH-generating system at 37°C for 15 min with rat liver microsomes and for 60 min with other microsomal preparations. Values are means ± S.D.

Microsomes from
n
Percentage Change in NNAL Quantity without CO
Percentage of (R)-NNAL
with CO
without CO
Human liver 3 −24 ± 12 74 ± 5 74 ± 6
Human lung 2 −10; + 4 90; 90 90; 88
Rat livera 4 −19 ± 4b 28 ± 1.5 25 ± 2.5c
Rat lunga
3
−7 ± 4
46 ± 2
47 ± 1.5

  • ↵ a Male Sprague-Dawley rats.

  • ↵ b P < 0.01 vs. samples with CO (paired t test, two-tailed).

  • ↵ c P < 0.05 vs. samples with CO (paired t test, two-tailed).