Primers for amplification and sequencing of UGT2B4 and UGT2B7
Gene | Step | Direction | Site | Primer Name | Sequence (5′ to 3′) |
|---|---|---|---|---|---|
| UGT2B4 | First Amplification | Forward | UGT2B4ZF | ATTGACTCTTACGGTAGAAGCAC | |
| Reverse | UGT2B4ZR | GTAGAAACCAGGTAGGAGTTGC | |||
| Second Amplification | Forward | PPREa | UGT2B4proF1 | GATAAAATCAGTAGGAGTTGGAAA | |
| Reverse | PPREa | UGT2B4proR1 | GTGTTGATTCTTACAACCATCAT | ||
| Forward | Exon1 | UGT2B4Ex1-1stF | TAAACGCACTTGTGTAAACAGG | ||
| Reverse | Exon1 | UGT2B4Ex1-1stR | AAATACCCCACTACCCTGACTT | ||
| Forward | Exon2 | UGT2B4Ex2-1stF | CTTCTTTCAGAAAATTACCTAGTTT | ||
| Reverse | Exon2 | UGT2B4Ex2-1stR | TGCTTTACTCTTCCCACTTCC | ||
| Forward | Exon3 | UGT2B4Ex3F | ACAATTCTTTCACAGTGCTTGC | ||
| Reverse | Exon3 | UGT2B4Ex3R | ACCAATGAAAAACACCTTCTGT | ||
| Forward | Exon4 | UGT2B4Ex4newF | ACATCAGTCTGAGTAGTTCTTGTT | ||
| Reverse | Exon4 | UGT2B4Ex4newR | CTCTGACTCTGATTGTAAAGAATG | ||
| Forward | Exon5 | UGT2B4Ex5F | ATCTAGGAAACACCGTCACATT | ||
| Reverse | Exon5 | UGT2B4Ex5R | AATAACCACTCAAAAATAAAAGCA | ||
| Forward | Exon6 | UGT2B4Ex6F | ATCTCCTGACCTCGTGATCC | ||
| Reverse | Exon6 | UGT2B4Ex6R | TGTCACAAGAAGAAAGGAATCTC | ||
| Sequencing | Forward | PPRE | UGT2B4proF1 | GATAAAATCAGTAGGAGTTGGAAA | |
| Reverse | PPRE | UGT2B4proRseq1 | AAACAAAAGTAAACCAAGCCTGT | ||
| Forward | Exon1 | UGT2B4Ex1-1stF | TAAACGCACTTGTGTAAACAGG | ||
| Forward | Exon1 | UGT2B4Ex1-Fseq2 | GATGAACTTGTCCAGAGAGGTC | ||
| Forward | Exon1 | UGT2B4Ex1-Fseq3 | AGATGCTGTTTTCCCCTTTG | ||
| Reverse | Exon1 | UGT2B4Ex1-Rseq1 | TAAGAGTAGATGGGCTGTTGG | ||
| Reverse | Exon1 | UGT2B4Ex1Rseq2-3 | ACAACAGGCACATAGGAAGG | ||
| Reverse | Exon1 | UGT2B4Ex1-1stR | AAATACCCCACTACCCTGACTT | ||
| Forward | Exon2 | UGT2B4Ex2-Fseq1 | TTTTTTTCCCCCATCAGGA | ||
| Reverse | Exon2 | UGT2B4Ex2-1stR | TGCTTTACTCTTCCCACTTCC | ||
| Forward | Exon3 | UGT2B4Ex3F | ACAATTCTTTCACAGTGCTTGC | ||
| Reverse | Exon3 | UGT2B4Ex3R | ACCAATGAAAAACACCTTCTGT | ||
| Forward | Exon4 | UGT2B4Ex4newF | ACATCAGTCTGAGTAGTTCTTGTT | ||
| Reverse | Exon4 | UGT2B4Ex4newR | CTCTGACTCTGATTGTAAAGAATG | ||
| Reverse | Exon4 | UGT2B4Ex4Rseq-he | TTTCATTTTATTTTTAAGTTTTTCC | ||
| Forward | Exon5 | UGT2B4Ex5F | ATCTAGGAAACACCGTCACATT | ||
| Reverse | Exon5 | UGT2B4Ex5R | AATAACCACTCAAAAATAAAAGCA | ||
| Forward | Exon6 | UGT2B4Ex6Fseq | GCTGGGATTGCAGGTGTG | ||
| Reverse | Exon6 | UGT2B4Ex6R | TGTCACAAGAAGAAAGGAATCTC | ||
| UGT2B7 | First Amplification | Forward | UGT2B7ZF2 | ATCTTGATTTCATAACTGTCATT | |
| Reverse | UGT2B7ZtaqR | GTAGTGTTTTCTTCATTGCCAC | |||
| Second Amplification | Forward | Promoter | UGT2B7proF1 | AAGTTTACAAAAATATGTGGACC | |
| Reverse | Promoter | UGT2B7proR2 | TATTAGCAAAATTACTGAAGTCC | ||
| Forward | Exon1 | UGT2B7Ex1F1st2seq1 | AAGGGTTACATTTTAACTTCTTGG | ||
| Reverse | Exon1 | UGT2B7Ex1R1st1seq3 | ATTCACTTACCAAAACCCCACT | ||
| Forward | Exon2 | UGT2B7Ex2-1stF2 | GATATTTGCCTACATTTTTGCC | ||
| Reverse | Exon2 | UGT2B7Ex2-1stR2 | CCCTTTGTAAATATTATTTGATTGG | ||
| Forward | Exon3 | UGT2B7Ex3Fseq1 | TCCAATAATTCCTCAAAATACTG | ||
| Reverse | Exon3 | UGT2B7Ex3-Rseq2 | CTCATAGTTTCTCCAAGATTATCC | ||
| Forward | Exon4 | UGT2B7Ex4-1stF3 | GCCACACGTAGGTTTTCTTTT | ||
| Reverse | Exon4 | UGT2B7Ex4Rseq1 | ATAAAGATTCCCCCGATTCAGA | ||
| Forward | Exon5 | UGT2B7Ex5-1stF3 | ACCGTATAGCCTTCAGTTACATAC | ||
| Reverse | Exon5 | UGT2B7Ex5-1stR4 | AGAAAATGGTTATACTCTGAGGTG | ||
| Forward | Exon6 | UGT2B7Ex6F | TTCTTTAACTCGGTGTCTGAGGG | ||
| Reverse | Exon6 | UGT2B7Ex6R | TGGAATAAACTGAAGTAGTCTCAC | ||
| Sequencing | Forward | Promoter | UGT2B7proF1 | AAGTTTACAAAAATATGTGGACC | |
| Reverse | Promoter | UGT2B7proR2 | TATTAGCAAAATTACTGAAGTCC | ||
| Forward | Exon1 | UGT2B7Ex1seqF1-4 | TTTATCTTTGGACATAACCATGA | ||
| Forward | Exon1 | UGT2B7Ex1seqF2 | GACTGTACTGGCATCTTCAGC | ||
| Forward | Exon1 | UGT2B7Ex1seqF3 | ATGCTATTTTTCCCTGTAGTGAG | ||
| Reverse | Exon1 | UGT2B7Ex1Rseq1-2 | TGACCATCTCTTAATCTGTTGC | ||
| Reverse | Exon1 | UGT2B7Ex1seqR2 | CGTAGGAAGGAGGGAAAATAA | ||
| Reverse | Exon1 | UGT2B7Ex1R1st1seq3 | ATTCACTTACCAAAACCCCACT | ||
| Forward | Exon2 | UGT2B7Ex2Fseq2 | TTTTTTCTATTCCTGTCAGGAAG | ||
| Reverse | Exon2 | UGT2B7Ex2-Rseq2 | ACTCATAAAACTCATATACGTGTGA | ||
| Forward | Exon3 | UGT2B7Ex3Fseq1 | TCCAATAATTCCTCAAAATACTG | ||
| Reverse | Exon3 | UGT2B7Ex3-Rseq2 | CTCATAGTTTCTCCAAGATTATCC | ||
| Forward | Exon4 | UGT2B7Ex4-1stF3 | GCCACACGTAGGTTTTCTTTT | ||
| Reverse | Exon4 | UGT2B7Ex4Rseq1 | ATAAAGATTCCCCCGATTCAGA | ||
| Reverse | Exon4 | UGT2B7Ex4heteroR | TTCTCCCTTAAGACTGGAAAATC | ||
| Forward | Exon5 | UGT2B7Ex5-1stF3 | ACCGTATAGCCTTCAGTTACATAC | ||
| Reverse | Exon5 | UGT2B7Ex5seqR2 | ATAAAAGCAGATTTCAGATTGGT | ||
| Forward | Exon6 | UGT2B7Ex6F | TTCTTTAACTCGGTGTCTGAGGG | ||
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| Reverse | Exon6 | UGT2B7Ex6Rseq1 | GTCTCACCTATCAGGTTTTCCA |
↵ a Peroxisome proliferator-activated receptor responsive element (PPRE) was used for the direct amplification of genomic DNA.