Primer sequences for RT-qPCR assays
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| Human | ||
| OATP-A/SLCO1A2 | TGGGGAACTTTGAAATGTGG | AAGGCTGGAACAAAGCTTGA |
| OATP-B/SLCO2B1 | TGATTGGCTATGGGGCTATC | CATATCCTCAGGGCTGGTGT |
| OATP-C/SLCO1B1 | GCCCAAGAGATGATGCTTGT | ATTGAGTGGAAACCCAGTGC |
| OATP8/SLCO1B3 | GGGTGAATGCCCAAGAGATA | ATTGACTGGAAACCCATTGC |
| NTCP/SLC10A1 | GGGACATGAACCTCAGCATT | CGTTTGGATTTGAGGACGAT |
| OAT2/SLC22A7 | GAGGATGAACCTGCCACAGT | TCTGCTCACACACCAGATCC |
| OCT1/SLC22A1 | TAATGGACCACATCGCTCAA | AGCCCCTGATAGAGCACAGA |
| 18S | CGCCGCTAGAGGTGAAATTC | TTGGCAAATGCTTTCGCTC |
| Rat | ||
| Oatp1/Slco1a1 | AGGTCGAGAATGACGGAGAA | ATTCCGAGGAAGGGAAGTGT |
| Oatp2/Slco1a4 | ATCTCGCTACCCCATTTCCT | TGGGATCTGTTTTCCACACA |
| Oatp4/Slco1b2 | GACATCACCCACTGGACCTT | AGCATGTTCCCCATCAAGAC |
| Ntcp/Slc10a1 | CAAGTCCAAAAGGCCACACT | TGATGACAGAGAGGGCTGTG |
| Oat2/Slc22a7 | CGCTCAGAATTCTCCTCCAC | GCACACCAATGAAGAAGCAG |
| Oct1/Slc22a1 | GGTTGCCAACAGGAGTCAGT | AGACAGAGCTTACGGCCAAA |
| 18S | CGAAAGCATTTGCCAAGAAT | AGTCGGCATCGTTTATGGTC |