Primers used in quantitative RNA-PCR Primer pairs for ALDH6, ALDH7, and CYP4B1 were the same as in Table 1. The accession number for β-actin is BC009275.
Primer | Sequence (5′-3′) | Size of PCR Product | Annealing Temperature | Position (5′) |
|---|---|---|---|---|
| bp | °C | |||
| ALDH6F2274 | agcccagagagcttag | 405 | 67 | 2274 |
| ALDH6R2678 | tgaagaccgaggtcct | 2678 | ||
| ALDH7F2245 | aggattgagggattgaggg | 510 | 65 | 2245 |
| ALDH7R2754 | agagagacaggagacggaac | 2754 | ||
| β-actinE3F | cctgactgactacctcatg | 396 | 60 | 1105 |
| β-actinE4R | tccttctgcatcctgtcggca | 1500 | ||
| CYP1B1F1 | cccagtcgtcatcagat | 221 | 62 | 4304 |
| CYP1B1R1 | cattctctctccggtaga | 4524 | ||
| CYP2F1F1 | gattctacggaatttcggg | 266 | 62 | 445 |
| CYP2F1R1 | tgtcgtacaactcgcc | 710 | ||
| CYP4B1F | taaggcccctgatgtgtatg | 369 | 60 | 339 |
| CYP4B1R | tggtactggaaggacacaag | 707 | ||
| FMO1F1363 | tgtgctactgcaaggc | 227 | 60 | 1363 |
| FMO1R1589 | cttgaatgttcggtccc | 1589 | ||
| GSTp1F1 | agtcttcgccaccatgccg | 375 | 60 | 1 |
| GSTp1R1 | ttcacatagtcatccttgcccac | 375 | ||
| UGT2A1F78 | ttggccaatggaaggtagtc | 208 | 60 | 138 |
| UGT2A1R285 | ccatgtcgaaacgaagtcct |
|
| 345 |