TABLE 1

Estimated values of kinetic model parameters

The kinetic model parameters Vmax21, Km21, V2, PS, k23, k32, Ki1, and Ki2 were estimated by fitting the solutions of eqs. 1 to 3 to data sets in Figs. 2 and 4. kpgp, kin, and kout were calculated from the kinetic model parameters as shown in the table. The parameter values are the mean ± the 95% confidence intervals.


Model Parameters

Estimated Values

95% Confidence Interval
Vmax21 (nmol/min) 56.6 ±14.4
Km21V2 (nmol) 39.4 ±20.0
kpgp = Vmax21/(Km21V2) (min−1) 1.44 ±0.72
kin = PS/V1 (min−1) 0.093 ±0.01
kout = PS/V2 (min−1) 0.011 ±0.02
k23 (min−1) 0.23 ±0.15
k32 (min−1) 0.006 ±0.02
Ki1/λ (μM) 0.36 ±0.29
Ki2/λ (μM)
1.23
±1.3