TABLE 1

Reduction of TNO by Hb-forming tamoxifen Conditions are as described under Materials and Methods. The incubations contained 2 mM NADPH and [3H]TNO (100,000 dpm, 50 nmol) in a volume of 0.5 ml. Data represent mean values ± S.D. of TAM formed (in nanomolars).

Amounts of Hb/Incubation
Period of Incubation
I. Hb
II. Hb + Reductase
III. Hb + NADPH
IV. Hb + NADPH + Reductase
min
50 pmol 0 1.1 ± 0.9 1.0 ± 0.9 6.3 ± 1.8 2.8 ± 0.3
30 2.0 ± 0.2 1.1 ± 1.0 6.0 ± 0.9* 11.9 ± 1.4**
100 pmol 0 1.5 ± 0.0 2.3 ± 0.9 8.2 ± 1.3 4.5 ± 1.2
30
1.1 ± 0.9
2.1 ± 0.4
8.1 ± 0.9*
28.2 ± 2.9**

  • ↵* Significant difference compared with groups I and II (P ≤ 0.05)

  • ↵** Significant difference compared with groups I, II, and III (P ≤ 0.05)