Incubation conditions for substrates in suspensions and monolayer cultures of rat hepatocytes
Substrate | Metabolite | Substrate Concentration | Suspensions | Monolayer Cultures | ||||
|---|---|---|---|---|---|---|---|---|
| Incubation Time | Cell Density | Incubation Time | Cell Density | |||||
| μM | min | 106cells/ml | min | 106cells/ml | ||||
| TOL | HTOL | 100-1500 | 15 | 1 | 90 | |||
| 7-EC | 7-HC | 0.05-200 | 15 | 0.5 | 60 | |||
| PHE | 4-OH PHE | 0.1-100 | 15 | 1 | 60 | |||
| DZ | 4′-OH DZ | 2.5-250 | 10 | 0.5 | 30 | |||
| NDZ | ||||||||
| TZ | ||||||||
| DEX | DOR | 1-500 | 10 | 0.5 | 60 | 1.9 | ||
| MEM | ||||||||
| WAR | 4-OH WAR | 25-1500 | 60 | 2 | 60 | |||
| 6-OH WAR | ||||||||
| 7-OH WAR | ||||||||
| 8-OH WAR | ||||||||
| 10-OH WAR | ||||||||
| PROP | - | 5 | Up to 60 | 0.2, 1 | Up to 120 |
| ||
-, PROP CLint determined by the substrate depletion method.