Effects of flavonoids on the uptake of [3H]DHEAS in both OATP1B1-expressing and OATP1B1-negative cells
The uptake of [3H]DHEAS (0.5 μM) in both OATP1B1-expressing and OATP1B1-negative cells in the absence or presence of flavonoids (50 μM) was performed as described under Materials and Methods. Rifampicin (100 μM) was used as a positive control. The uptake of [3H]DHEAS was expressed as the percentage of control (uptake in OATP1B1-negative cells in the presence of 0.3% DMSO). The data are expressed as mean ± S.D., n ≥ 6. * p < 0.05, ** p < 0.01 compared with the control of either OATP1B1-expressing or OATP1B1-negative cells. The values in parentheses represent the percentage of decrease or increase in means relative to the control value for either OATP1B1-expressing or OATP1B1-negative cells.
OATP1B1-Expressing Cells | OATP1B1-Negative Cells | |||||
|---|---|---|---|---|---|---|
| Mean ± S.D. | (Perceentage Change in Mean) | Mean ± S.D. | (Percentage Change in Mean) | |||
| Control | 452 ± 105 | 100 ± 7.93 | ||||
| Rifampicin | 195 ± 78.5** | (-56.8) | 97.1 ± 10.9 | (-2.88) | ||
| Apigenin | 362 ± 49.8* | (-19.8) | 193 ± 42.3** | (+92.8) | ||
| Biochanin A | 142 ± 35.0** | (-68.6) | 90.1 ± 19.6 | (-9.95) | ||
| Benzoflavone | 445 ± 29.3 | (-1.53) | 112 ± 4.48 | (+11.9) | ||
| Chrysin | 477 ± 77.0 | (+5.53) | 117 ± 10.6** | (+17.5) | ||
| Fisetin | 197 ± 22.8** | (-56.5) | 138 ± 13.0** | (+37.9) | ||
| Galangin | 391 ± 19.6 | (-13.5) | 118 ± 9.49* | (+17.8) | ||
| Kaempferol | 378 ± 68.8 | (-16.5) | 85.9 ± 3.75 | (-14.1) | ||
| Luteolin | 217 ± 44.6** | (-52.0) | 118 ± 6.41** | (+17.8) | ||
| Morin | 252 ± 20.0** | (-44.2) | 87.7 ± 11.8 | (-12.3) | ||
| Myricetin | 305 ± 24.0** | (-32.5) | 94.0 ± 10.5 | (-6.03) | ||
| Silibinin | 152 ± 15.2** | (-66.5) | 114 ± 5.70* | (+13.9) | ||
| Silymarin | 140 ± 11.6** | (-68.9) | 96.0 ± 12.8 | (-3.96) | ||