Kinetic parameters of MPAG and AcMPAG formation derived from incubations with recombinant human UGT isoforms (Supersomes)
UGT Isoform | MPAG Formation Kinetic Parameter | AcMPAG Formation Kinetic Parameters | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Km | Vmax | CLint | Km | Vmax | CLint | |||||
| mM | nmol/mg/min | μl/mg/min | mM | nmol/mg/min | μl/mg/min | |||||
| 1A1 | 0.41 | 0.11 | 0.26 | 0.32 | 0.03 | 0.08 | ||||
| 1A6 | LA | N.A. | ||||||||
| 1A7 | 0.48 | 7.14 | 14.88 | N.A. | ||||||
| 1A8 | 1.39 | 7.27 | 5.22 | N.A. | ||||||
| 1A9 | 0.16 | 11.82 | 73.86 | N.A. | ||||||
| 1A10 | LA | N.A. | ||||||||
| 2B4 | LA | L.A. | ||||||||
| 2B7 |
| LA |
| 0.20 | 0.22 | 1.08 | ||||
N.A., no activity; L.A., low activity.