Toxicokinetic parameters estimated for TA and TASO following intraperitoneal administration of 50, 300, and 600 mg/kg doses of TA
Plasma TA and TASO concentrations were determined using reversed-phase HPLC. Male Sprague-Dawley rats were treated with 50, 300, or 600 mg TA/kg via i.p. injection in saline vehicle, and plasma was collected via serial blood sampling over a time course of 0 to 1040 min. See Materials and Methods for detailed sample collection and HPLC procedure. Results are expressed as means ± S.E. (n = 3).
Parameter | Dose of Thioacetamide | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 50 mg/kg | 300 mg/kg | 600 mg/kg | |||||||||
| TA | TASO | TA | TASO | TA | TASO | ||||||
| tmax (min) | 5 | 90 | 5 | 180 | 5 | 180 | |||||
| Cmax (μg/ml) | 30.5 ± 2.7 | 19.6 ± 1.12 | 198 ± 27.3 | 50.2 ± 2.42 | 586.8 ± 31.29 | 77 ± 2.9 | |||||
| AUC (μg · min/ml) | 802 ± 9 | 2037 ± 71 | 26,509 ± 1421.6 | 15,443 ± 1631 | 141,460 ± 23,434 | 53,198 ± 3399 | |||||
| AUC/dose | 16 ± 2 | 40.7 ± 1.4 | 88.4 ± 4.7 | 51.5 ± 5.4 | 235.8 ± 39.1 | 88.7 ± 5.7 | |||||
| Apparent t1/2 (min) | 16 ± 1 | 36 ± 1.2 | 74.5 ± 4 | 81.4 ± 16.7 | 241.36 ± 21.6 | 247 ± 41.7 | |||||
| Plasma Clearance (1/kg · h) | 1.63 ± 0.21 | 0.82 ± 0.06 | 0.30 ± 0.01 | 0.15 ± 0.04 | 0.14 ± 0.01 | 0.04 ± 0.01 | |||||