TABLE 3

Primer sequences used for the PCR-SSCP analysis of the human BCRP gene


Primer Pair

Forward Primer

Reverse Primer
Promoter-1 5′-GTACTAGGATTACAGGCGTGAGC-3′ 5′-AATGACCCGAGATCCCACCACTG-3′
Promoter-2 5′-CGCAGATTTTTCAGTTTTTGC-3′ 5′-TATTAATGGTTCACCCAATGC-3′
Promoter-3 5′-AGGGTCTTGAAACTGACAGAAAT-3′ 5′-AAGGGGAGAAACTTACTGAATGA-3′
Promoter-4 5′-TTTCTAGCCTTTCCACACCATCG-3′ 5′-ACGCAGGGACAAGCCAAACACTC-3′
Promoter-5 5′-GCCAGTGACGGCGACCAAACC-3′ 5′-GCGCTGACACGAACTTCCTAAGC-3′
Exon 1-1 5′-GGCTCAGCGCGGCAGGACA-3′ 5′-GACCCGGACATCCAGGGGACGAG-3′
Exon 1-2 5′-AGCGCGGCTTAGGAAGTTCGTGT-3′ 5′-CGCGTCTCTCAATCTCAGTGG-3′
Exon 1-3 5′-CGTGCTGTGCCCACTCAAAAG-3′ 5′-ATACACAACGCCCACCAACCT-3′
Exon 2 5′-ATTGTCACCTAGTGTTTGC-3′ 5′-AAAAATGTTCATAGCCAGTTTCT-3′
Exon 3 5′-AAAATGGAATAGTAAATCAGTCT-3′ 5′-CTGCTACATGTCAATCACCTTAT-3′
Exon 4 5′-ATAGCATGTGTTGGAGGGAAAAA-3′ 5′-ATTGGTATCACTGTCCTTACAAG-3′
Exon 5 5′-AAACAGTCATGGTCTTAGAAAAG-3′ 5′-TCTCATTGTTATGGAAAGCAACC-3′
Exon 6 5′-CAAATGATAATGACTGGTTGTTA-3′ 5′-TTGTTTTTCTTGATAATGCTTTT-3′
Exon 7 5′-AGCAAACAATCTAAAGGCAAGAA-3′ 5′-CCAAAGACCAAACAGCACTCCTG-3′
Exon 8 5′-AAGTGAGTTCTCTTTGTTTTCCA-3′ 5′-GTTGACTGGTATCAGAAGACTGC-3′
Exon 9 5′-TGTTTGTGTTTCCTTTTTATCCA-3′ 5′-CATTGTTCCCATTTGAGTATTTC-3′
Exon 10 5′-TTATCTCTAATTGAAACTCTTCC-3′ 5′-AAATAAACTGACTCATCCTACCC-3′
Exon 11 5′-ACGCTTCCCTGTTCCAACCAGAA-3′ 5′-TGTAATCAGTCTAACCAATAGCC-3′
Exon 12 5′-GACAAGTCTAGCCTGCCCTGTGG-3′ 5′-GTTTGGTTTATAGTTTTGAGAAC-3′
Exon 13 5′-AATAAGCAATCCCAAACATACGG-3′ 5′-TTATCAGAGCAAACACAGTTCAG-3′
Exon 14 5′-AGAGGAGAAGAGTTTAGTGAGTG-3′ 5′-ACAGTGACAGACAAGGAAGACAT-3′
Exon 15 5′-ACATTAGTTGGTTTGGTGAGACA-3′ 5′-ATTCAGTGCCCCTGGAAGGACTC-3′
Exon 16-1 5′-CTGAGTAACATTTGACGGATGCT-3′ 5′-GTGATGGCAAGGGAACAGAAAAC-3′
Exon 16-2 5′-TTCAGTATGATTTATCCTCACAT-3′ 5′-GCTACTAACCTACCTATTCATTT-3′
Exon 16-3
5′-GTCTCTGGCATTTGTTTCCTCAT-3′
5′-CTCTACTCTACCCACAGTTCCAA-3′