TABLE 1

Kinetic parameters of AFQ N-glucuronidation in liver microsomes from humans and experimental animals

The kinetic parameters were calculated with GraphPad Prism software. Each value represents best-fit values ± S.E. of triplicate points. Kinetic plots are presented in Fig. 3.

Species	K_m or S_5₀	V_max	CL_int^a or CL_max^b
	μM	pmol/min/mg protein	μl/min/mg protein
Human^c	2019 ± 85.9	871.2 ± 17.9	0.432
Rat^c	611.0 ± 26.3	89.2 ± 1.3	0.146
Dog^d	582.1 ± 13.2	236.9 ± 2.4	0.219
Monkey^c	732.5 ± 47.2	90.1 ± 2.1	0.123
Rabbit^c	24.8 ± 0.7	9375 ± 68.9	378.2

↵ a CL_int calculated as V_max/K_m for Michaelis-Menten kinetics.
↵ b CL_max calculated as described under Materials and Methods.
↵ c Michaelis-Menten.
↵ d Hill equation (n = 1.45 ± 0.033).