Effects of nicotine and NNN on the kinetic parameters of CYP2A13-catalyzed NNK metabolism
Values in the parentheses were obtained from our previous report (Su et al., 2000), which agree well with the present result.
Km | Vmax | |
|---|---|---|
| μM | pmol/min/pmol | |
| P450 | ||
| Keto aldehyde formation | ||
| NNK | 10.4 (11.3 ± 3.5) | 3.6 (4.1 ± 0.6) |
| NNK + NNN | 52.6 | 3.7 |
| NNK + nicotine | 43.5 | 3.7 |
| Keto alcohol formation | ||
| NNK | 8.8 (13.1 ± 5.1) | 0.9 (1.2 ± 0.1) |
| NNK + NNN | 60.5 | 1.2 |
| NNK + nicotine | 55.5 | 1.4 |