Tritium isotope effect for hydroxylation of [6β-3H]testosterone
Formation of tritiated water and 6β-hydroxytestosterone was determined at nine different time points was between 2 and 30 min, using four different concentrations of HLMs. 6β-Hydroxytestosterone was quantified by LC-MS/MS analysis. For each microsome concentration, data are mean values from all the time points.
Microsome Concentration | SA0/SAP | TV/K |
|---|---|---|
| mg/ml | ||
| 0.1 | 2.39 | 2.40 |
| 0.25 | 2.22 | 2.24 |
| 0.5 | 2.18 | 2.21 |
| 1.0 | 2.47 | 2.53 |
| Average ± S.D. | 2.32 ± 0.14 | 2.34 ± 0.15 |
SA0, initial specific radioactivity of [6β-3H]testosterone; SAP, specific radioactivity of the product, as defined under Materials and Methods; TV/K, kinetic isotope effect.