TABLE 3

Hepatocyte binding data (mean fu ± S.D. from three replicate measurements)


Compound

Hepatocytes Containing 0.2% BSA

Hepatocytes without BSA

Dead Hepatocytes without BSA (fuobserved)
fuobserved
fucorrected
fuobserved
fucorrected
2-Ethoxybenzamide 0.96 ± 0.02 0.95 ± 0.02 0.93 ± 0.01 0.90 ± 0.01 0.98 ± 0.01
Albendazole 0.52 ± 0.07 0.60 ± 0.07 0.54 ± 0.01 0.66 ± 0.01 0.50 ± 0.06
Astemizole 0.029 ± 0.0072 0.038 ± 0.0085 0.038 ± 0.016 0.056 ± 0.023 0.023 ± 0.010
Betaxolol 0.71 ± 0.06 0.70 ± 0.06 0.72 ± 0.07 0.70 ± 0.07 0.78 ± 0.03
Bumetanide 0.29 ± 0.02 0.28 ± 0.02 0.95 ± 0.06 0.91 ± 0.06 0.86 ± 0.06
Cerivastatin 0.41 ± 0.1 0.40 ± 0.1 0.69 ± 0.01 0.67 ± 0.01 0.71 ± 0.02
Clozapine 0.24 ± 0.04 0.23 ± 0.04 0.51 ± 0.04 0.50 ± 0.04 0.22 ± 0.06
Diazepam 0.66 ± 0.09 0.65 ± 0.09 0.82 ± 0.03 0.81 ± 0.03 0.87 ± 0.01
Glyburide 0.040 ± 0.004 0.041 ± 0.004 0.67 ± 0.01 0.60 ± 0.01 0.46 ± 0.02
Indapamide 0.75 ± 0.04 0.75 ± 0.04 0.83 ± 0.01 0.82 ± 0.01 0.87 ± 0.01
Isradipin 0.26 ± 0.04 0.25 ± 0.03 0.28 ± 0.02 0.28 ± 0.02 0.26 ± 0.03
Metoprolol 0.84 ± 0.08 0.82 ± 0.08 0.97 ± 0.03 0.95 ± 0.03 0.96 ± 0.02
Metyrapone 0.72 ± 0.07 0.65 ± 0.07 0.88 ± 0.04 0.77 ± 0.04 0.95 ± 0.02
Omeprazole 0.81 ± 0.02 0.80 ± 0.02 0.94 ± 0.02 0.93 ± 0.02 0.86 ± 0.03
Oxaprozin 0.30 ± 0.10 0.29 ± 0.10 0.85 ± 0.02 0.83 ± 0.02 0.66 ± 0.05
Propranolol 0.68 ± 0.05 0.67 ± 0.05 0.54 ± 0.08 0.53 ± 0.08 0.50 ± 0.01
Verapamil
0.49 ± 0.1
0.42 ± 0.09
0.69 ± 0.06
0.63 ± 0.05
0.69 ± 0.11