Kinetic parameters of SN-38 glucuronidation by wild-type and P229L UGT1A1
Parameter | Km | Vmax | Vmax/Km | Normalized Vmax | Normalized Vmax/Km |
|---|---|---|---|---|---|
| UGT1A1 | μM | pmol/min/mg protein | ml/min/mg protein | pmol/min/mg protein | ml/min/mg protein |
| Wild-type | 8.67 ± 0.18 | 71.2 ± 4.73 | 8.23 ± 0.65 | 71.7 ± 2.46 | 8.26 ± 0.15 |
| P229L | 37.6 ± 2.34 | 5.27 ± 0.32 | 0.14 ± 0.02 | 8.93 ± 0.98 | 0.24 ± 0.03 |