Functional activity (mean ± S.D.) of ABCB1 and ABCG2 measured as uptake of vinblastine and mitoxantrone, respectively, in microvillus membrane vesicles CYP1A1 activity (mean ± S.D.) was measured as ethoxyresorufin -O-deethylation in placental microsomes.
Parameter (Units) | Smokers (n = 10) | Nonsmokers (n = 10) |
|---|---|---|
| ABCB1-mediated vesicular uptake of vinblastine (pmol/mg protein) | 2.25 ± 1.12 | 1.72 ± 0.67 |
| ABCG2-mediated vesicular uptake of mitoxantrone (pmol/mg protein) | 6.25 ± 1.55 | 5.53 ± 2.09 |
| CYP1A1 activity in placental microsomes (pmol/min/mg protein) | 3.61 ± 2.96 | 0.56 |
|
|
| (0.19, 0.93)a |
↵ a Measurable values: 8 of the 10 samples were below the limit of detection