Cytotoxicities of St. John's wort extract, hyperforin, and hypericin in LLC-PK1 and LLC-GA5-COL150 cells
Treatment and Concentration | LLC-PK1 | GA5-COL150 | LLC-PK1 | GA5-COL150 | ||
|---|---|---|---|---|---|---|
| 90 min | 180 min | |||||
| St. John's wort extract | ||||||
| 10 μg/ml | O | – | O | – | ||
| 20 μg/ml | O | O | O | O | ||
| 50 μg/ml | X | O | X | X | ||
| 75 μg/ml | X | O | X | X | ||
| Hyperforin | ||||||
| 0.2 μM | – | – | O | – | ||
| 0.5 μM | O | O | O | O | ||
| 1.0 μM | O | O | – | X | ||
| Hypericin | ||||||
| 0.01 μM | – | O | – | O | ||
| 0.02 μM | – | O | – | O | ||
| 0.05 μM | – | O | – | O | ||
| 0.1 μM | O | O | O | O | ||
| 0.2 μM | – | Δ | – | X | ||
| 0.5 μM | – | Δ | – | X | ||
O, ≥90%, Δ, 80–90% but not statistically significant; X, significantly reduced; –, not tested.