Parent ions and major fragment ions of clofarabine and authentic reference metabolites following LC-MS/MS analyses under negative mode of detection In parenthesis are metabolites observed from in vitro hepatocyte studies. The most intense ions are presented in bold.
Peak | Description | (M–H)-m/z | Major Fragment Ions |
|---|---|---|---|
| 1–6 | Unknown | N.S. | N.A. |
| 7 | 2-Chloroadenine | 168 | 132 |
| 8 | Unknown (rat) | 343 | N.D. |
| 9a | 6-Ketoclofarabine | 303 | 133, 169, 189, 225, 235, 265, 283 |
| 9b | Clofarabine phosphate | 382 | 79, 168, 195, 213 |
| 9c | Unknown (rat) | 392 | N.D. |
| 10 | Unknown | N.D. | N.D. |
| 11 | Carboxy- or methoxy-clofarabine | 316 | 132, 168, 198 |
| 12 | Clofarabine-glucuronide (rat and dog) | 478 | N.D. |
| 13a | Clofarabine-sulfate | 382 | 168, 213, 362 |
| 13b | Clofarabine-glucuronide (rat) | 478 | N.D. |
| 14 | Clofarabine-sulfate (rat, dog, human) | 382 | 168, 213, 362 |
| 15a | Clofarabine | 302 | 132, 168, 188, 198, 224, 228, 234, 246, 264, 282 |
| 16 | Unknown | N.S. | N.A. |
| 17 | Unknown | N.S. | N.A. |
N.S., not studied; N.A., not applicable; N.D., not detected.
↵ a Denotes that peak is parent clofarabine