TABLE 3

Quantity of individual radiolabeled metabolites in rat plasma and tissues after oral ingestion of [2-14C]quercetin-4′-glucoside

Radiolabeled metabolites identified using HPLC-RC-MS/MS. Quantity of each metabolite expressed in dpm × 103 per whole organ or total plasma volume (100 × 103 dpm = 12 pmol of radiolabeled compound).

Peak
Compound
0.5 h
1 h
2 h
5 h
GIT
Plasma
Liver
Kidneys
GIT
Plasma
Liver
Kidneys
GIT
Plasma
Liver
Kidneys
GIT
Plasma
Liver
Kidneys
1 Not identified 5716
2 Not identified 5824
3 Quercetin diglucuronide 43 38 437 44 35 26 55 66
4 Not identified 905 20 437 21 1545 7 6
5 Methylated quercetin diglucuronide 131 94 710 89 79 2832 15 16
6 Quercetin diglucuronide 20 18 109 105 1493 142 9 1294 77 66 28
7 Quercetin diglucuronide 75 31
8 Methylated quercetin diglucuronide 77 72 328 58 3708 4 1132 4
9 Quercetin diglucuronide 105 32 61 79 21 17 31
10 Quercetin diglucuronide 1107 509 169 8 1365 337 229 55 7673 218 91 20 35967 21 13 55
11 Quercetin diglucuronide 2264 180 80 35 328 135 27 39 1854 83 23 4 18
12 Quercetin-3-glucuronide 156 35 5786 35 47 37 4428
13 Quercetin glucuronide sulfate 27 77 3766 227 20 3605 245 25 647 138
14 Methylated quercetin diglucuronide 56
15 Quercetin monoglucuronide 271
16 Methylated quercetin glucuronide sulfate 159 253 14 382 717 87 14 3656 694 61 611
17 Quercetin glucuronide sulfate 56 3 2
18 Quercetin-4′-glucoside 24648 20 14301
19 Methylated quercetin monoglucuronide 19 118 54 3548 8 92 36 3708 25 8 4
20 Methylated quercetin monoglucuronide 1258 36 93 133 2675 7 38 79 3605 12 9
21 Methylated quercetin monoglucuronide 44 66 42 4737 22
6659* 13* 14*
22 Quercetin monoglucuronide 1660
23 Quercetin monoglucuronide 39
24 Quercetin 12626 3930 10 3450 3289 7 3
25 Methylated quercetin sulfate
9971**
26 Quercetin sulfate 3270 4686
27
Isorhamnetin
2565
515

  • GIT, gastrointestinal tract.

  • ↵* Peaks 21 and 22 indistinguishable in these samples.

  • ↵** Peaks 25 and 26 indistinguishable in this sample.